Genomic Library- A detailed note

It is the collection of total genomic DNA from a single organism. The total genome from an organism is taken and digested into fragments. Then, they are carried into a host organism by a vector DNA and cloned into many copies. Through screening process, the cloned host organism with the gene of interest is taken and preserved for future use.

So, now we will discuss the steps in constructing a genomic library.

1) Isolation and purification of total genomic DNA from the cell (Prokaryote or eukaryote)

CLICK HERE TO READ

2) Restriction digestion of the total genomic DNA using Restriction enzymes.

CLICK HERE TO READ

3) Selection of Vector

CLICK HERE TO READ

4) Insertion of the fragment into the vector using Ligase enzyme.

CLICK HERE TO READ

5) Host Transformation.

CLICK HERE TO READ

6) Selection. 

CLICK HERE TO READ