11 September 2013

cDNA Library - A detailed note

Its a collection of  cDNA (Complementary DNA) clones generated from mRNA sequences of  a single cell population. cDNA is double stranded DNA complementary to the mRNA sequence synthesized by the enzymes reverse transcriptase and DNA polymerase. 

Steps involved in creating cDNA library: 

Extraction and purification of mRNA from the cell

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Synthesis of cDNA from the total mRNA

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cDNA cloning

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Screening of  the host containing the cDNA 

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