It is the collection of
total genomic DNA from a single organism. The total genome from an organism is
taken and digested into fragments. Then, they are carried into a host organism
by a vector DNA and cloned into many copies. Through screening process, the
cloned host organism with the gene of interest is taken and preserved for
future use.
1) Isolation and
purification of total genomic DNA from the cell (Prokaryote or eukaryote)
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2) Restriction
digestion of the total genomic DNA using Restriction enzymes.
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3) Selection of Vector
4) Insertion of the
fragment into the vector using Ligase enzyme.
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5) Host Transformation.
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6) Selection.
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