Its a collection of cDNA (Complementary DNA) clones generated from mRNA sequences of a single cell population. cDNA is double stranded DNA complementary to the mRNA sequence synthesized by the enzymes reverse transcriptase and DNA polymerase.
Steps involved in creating cDNA library:
Extraction and purification of mRNA from the cell
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Synthesis of cDNA from the total mRNA
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cDNA cloning
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Screening of the host containing the cDNA
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